› 7z0o

Electron Microscopy
2.8Å resolution

Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA

Released:
DOI: 10.2210/pdb7z0o/pdb
PDB entry 7z0o coloured by chain, front view.
PDB entry 7z0o coloured by chain, side view.
PDB entry 7z0o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mechanism of RNA polymerase I selection by transcription factor UAF.
Baudin F, Murciano B, Fung HKH, Fromm SA, Mattei S, Mahamid J, Müller CW
OpenAccess logo Sci Adv 8 eabn5725 (2022)
PMID: 35442737
Related structures: EMD-14428

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-122360 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
Histone H3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 136 amino acids
Theoretical weight: 15.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61830 (Residues: 1-136; Coverage: 100%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 103 amino acids
Theoretical weight: 11.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02309 (Residues: 1-103; Coverage: 100%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W
Sequence domains: Centromere kinetochore component CENP-T histone fold
RNA polymerase I-specific transcription initiation factor RRN5 Chain: D
RNA polymerase I-specific transcription initiation factor RRN9 Chain: E
RNA polymerase I-specific transcription initiation factor RRN10 Chain: F
Upstream activation factor subunit UAF30 Chain: G
TATA-box-binding protein Chain: H
Non-template DNA Chain: N
Template DNA Chain: T

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-14428
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Transcription elongation mechanisms of RNA polymerases I, II, and III and their therapeutic implications.
Jacobs et al. (2024)
2 other articles

1 mention without citation

Ribosome biogenesis factors-from names to functions.
Dörner et al. (2023)

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 7z0o found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-10964
Datasets: 2
Total size: 3100Gb
EMPIAR thumbnail