› 7z0z

Electron Microscopy
2.68Å resolution

Abortive infection DNA polymerase AbiK from Lactococcus lactis, Y44F variant

Released:
DOI: 10.2210/pdb7z0z/pdb
PDB entry 7z0z coloured by chain, front view.
PDB entry 7z0z coloured by chain, side view.
PDB entry 7z0z coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.
Figiel M, Gapińska M, Czarnocki-Cieciura M, Zajko W, Sroka M, Skowronek K, Nowotny M
OpenAccess logo Nucleic Acids Res (2022)
PMID: 36107766
Related structures: EMD-14435

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse transcriptase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 601 amino acids
Theoretical weight: 71.62 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q48614 (Residues: 1-599; Coverage: 100%)
Gene name: abiK
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.68Å
Relevant EMDB volumes: EMD-14435
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase-Cas1 fusion protein.
Mohr et al. (2024)
1 more

1 mention without citation

Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.
Figiel et al. (2022)