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Abortive infection DNA polymerase AbiK from Lactococcus lactis, Y44F variant

Electron Microscopy
2.68Å resolution
PDB entry 7z0z coloured by chain, front view.
PDB entry 7z0z coloured by chain, side view.
PDB entry 7z0z coloured by chain, top view.
Source organism: Lactococcus lactis
Assembly composition:
homo hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Reverse transcriptase domain-containing protein
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Multimeric state: homo hexamer
Accessible surface area: 130585.62 Å2
Buried surface area: 23902.63 Å2
Dissociation area: 4,178.72 Å2
Dissociation energy (ΔGdiss): 7.56 kcal/mol
Dissociation entropy (TΔSdiss): 18.11 kcal/mol
Symmetry number: 6
PDBe Complex ID: PDB-CPX-175366

Macromolecules

Reverse transcriptase domain-containing protein
Chains: A, B, C, D, E, F
Length: 601 amino acids
Theoretical weight: 71.62 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q48614 (Residues: 1-599; Coverage: 100%)
Gene name: abiK
Pfam: Reverse transcriptase (RNA-dependent DNA polymerase)
InterPro:
Reverse transcriptase domain-containing protein in PDB entry 7z0z, assembly 1, front view.
Reverse transcriptase domain-containing protein in PDB entry 7z0z, assembly 1, side view.
Reverse transcriptase domain-containing protein in PDB entry 7z0z, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q48614  
160150100150200250300350400450500550600
 
200400600GSMKKEFTELYDFIFDPIFLVRYGYYDRSIKNKKMNTAKVELDNEFGKSDSFYFKVFNMESFADYLRSHDLKTHFNGKKPLSTDPVYFNIPKNIEARRQYKMPNLYSYMALNYYICDNKKEFIEVFIDNKFSTSKFFNQLNFDYPKTQEITQTLLYGGIKKLHLDLSNFYHTLYTHSIPWMIDGKSASKQNRKKGFSNTLDTLITACQYDETHGIPTGNLLSRIITELYMCHFDKQMEYKKFVYSRYVDDFIFPFTFENEKQEFLNEFNLICRENNLIINDNKTKVDNFPFVDKSSKSDIFSFFENITSTNSNDKWIKEISNFIDYCVNEEHLGNKGAIKCIFPVITNTLKQKKVDTKNIDNIFSKRNMVTNFNVFEKILDLSLKDSRLTNKFLTFFENINEFGFSSLSASNIVKKYFSNNSKGLKEKIDHYRKNNFNQELYQILLYMVVFEIDDLLNQEELLNLIDLNIDDYSLILGTILYLKNSSYKLEKLLKKIDQLFINTHANYDVKTSRMAEKLWLFRYFFYFLNCKNIFSQKEINSYCQSQNYNSGQNGYQTELNWNYIKGQGKDLRANNFFNELIVKEVWLISCGENEDFKYLN
UniProt
Q48614
Chains
Domains
Secondary structure
Interaction interfaces
Sequence conservation
Molecule details ›

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