› 7z14

Electron Microscopy
3.15Å resolution

Cryo-EM structure of Torpedo nicotinic acetylcholine receptor in complex with a short-chain neurotoxin.

Released:
DOI: 10.2210/pdb7z14/pdb
PDB entry 7z14 coloured by chain, front view.
PDB entry 7z14 coloured by chain, side view.
PDB entry 7z14 coloured by chain, top view.
Source organisms:
Primary publication:
The molecular mechanism of snake short-chain α-neurotoxin binding to muscle-type nicotinic acetylcholine receptors.
Nys M, Zarkadas E, Brams M, Mehregan A, Kambara K, Kool J, Casewell NR, Bertrand D, Baenziger JE, Nury H, Ulens C
OpenAccess logo Nat Commun 13 4543 (2022)
PMID: 35927270
Related structures: EMD-14440

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136092 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (8 distinct):
Acetylcholine receptor subunit alpha Chains: A, D
Molecule details ›
Chains: A, D
Length: 437 amino acids
Theoretical weight: 50.17 KDa
Source organism: Tetronarce californica
UniProt:
  • Canonical: P02710 (Residues: 25-461; Coverage: 100%)
Gene name: CHRNA1
Sequence domains:
Acetylcholine receptor subunit beta Chain: B
Molecule details ›
Chain: B
Length: 469 amino acids
Theoretical weight: 53.73 KDa
Source organism: Tetronarce californica
UniProt:
  • Canonical: P02712 (Residues: 25-493; Coverage: 100%)
Gene name: CHRNB1
Sequence domains:
Acetylcholine receptor subunit delta Chain: C
Molecule details ›
Chain: C
Length: 501 amino acids
Theoretical weight: 57.63 KDa
Source organism: Tetronarce californica
UniProt:
  • Canonical: P02718 (Residues: 22-522; Coverage: 100%)
Gene name: chrnd
Sequence domains:
Acetylcholine receptor subunit gamma Chain: E
Molecule details ›
Chain: E
Length: 489 amino acids
Theoretical weight: 56.34 KDa
Source organism: Tetronarce californica
UniProt:
  • Canonical: P02714 (Residues: 18-506; Coverage: 100%)
Gene name: CHRNG
Sequence domains:
Consensus short-chain short-chain alpha-neurotoxin ScNtx Chains: F, G
Molecule details ›
Chains: F, G
Length: 60 amino acids
Theoretical weight: 6.85 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-14440
Expression system: Escherichia coli

Quick links

Citations

6 review citations

From squid giant axon to automated patch-clamp: electrophysiology in venom and antivenom research.
Ahmadi et al. (2023)
5 more