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Architecture of the yeast Pol III pre-termination complex and pausing mechanism on poly(dT) termination signals.

Girbig M, Xie J, Grötsch H, Libri D, Porrua O, Müller CW
Cell Rep 40 111316 (2022)
Related entries: 7z1l, 7z1m, 7z1n

Cited: 7 times
EuropePMC logo PMID: 36070694

Abstract

RNA polymerase (Pol) III is specialized to transcribe short, abundant RNAs, for which it terminates transcription on polythymine (dT) stretches on the non-template (NT) strand. When Pol III reaches the termination signal, it pauses and forms the pre-termination complex (PTC). Here, we report cryoelectron microscopy (cryo-EM) structures of the yeast Pol III PTC and complementary functional states at resolutions of 2.7-3.9 Å. Pol III recognizes the poly(dT) termination signal with subunit C128 that forms a hydrogen-bond network with the NT strand and, thereby, induces pausing. Mutating key interacting residues interferes with transcription termination in vitro, impairs yeast growth, and causes global termination defects in vivo, confirming our structural results. Additional cryo-EM analysis reveals that C53-C37, a Pol III subcomplex and key termination factor, participates indirectly in Pol III termination. We propose a mechanistic model of Pol III transcription termination and rationalize why Pol III, unlike Pol I and Pol II, terminates on poly(dT) signals.

Reviews citing this publication (1)

  1. CRISPR-Cas Technology for Bioengineering Conventional and Non-Conventional Yeasts: Progress and New Challenges. Xia Y, Li Y, Shen W, Yang H, Chen X. Int J Mol Sci 24 15310 (2023)

Articles citing this publication (6)

  1. Structural basis for intrinsic transcription termination. You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y. Nature 613 783-789 (2023)
  2. Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase. Vishwakarma RK, Qayyum MZ, Babitzke P, Murakami KS. Proc Natl Acad Sci U S A 120 e2218516120 (2023)
  3. Structural basis of Ty1 integrase tethering to RNA polymerase III for targeted retrotransposon integration. Nguyen PQ, Huecas S, Asif-Laidin A, Plaza-Pegueroles A, Capuzzi B, Palmic N, Conesa C, Acker J, Reguera J, Lesage P, Fernández-Tornero C. Nat Commun 14 1729 (2023)
  4. DNA-directed termination of RNA polymerase II transcription. Han Z, Moore GA, Mitter R, Lopez Martinez D, Wan L, Dirac Svejstrup AB, Rueda DS, Svejstrup JQ. Mol Cell 83 3253-3267.e7 (2023)
  5. DNA-dependent RNA polymerases in plants. Yang DL, Huang K, Deng D, Zeng Y, Wang Z, Zhang Y. Plant Cell 35 3641-3661 (2023)
  6. Transcription termination and readthrough in African swine fever virus. Cackett G, Sýkora M, Portugal R, Dulson C, Dixon L, Werner F. Front Immunol 15 1350267 (2024)


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