› 7z3r

X-ray diffraction
2.95Å resolution

Crystal structure of the mouse leptin:LepR-IgCRH2 complex to 2.95 A resolution.

Released:
DOI: 10.2210/pdb7z3r/pdb
PDB entry 7z3r coloured by chain, front view.
PDB entry 7z3r coloured by chain, side view.
PDB entry 7z3r coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mechanism of receptor assembly via the pleiotropic adipokine Leptin.
Tsirigotaki A, Dansercoer A, Verschueren KHG, Marković I, Pollmann C, Hafer M, Felix J, Birck C, Van Putte W, Catteeuw D, Tavernier J, Fernando Bazan J, Piehler J, Savvides SN, Verstraete K
Nat Struct Mol Biol (2023)
PMID: 36959263

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214911 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leptin Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41160 (Residues: 20-167; Coverage: 100%)
Gene names: Lep, Ob
Sequence domains: Leptin
Leptin receptor Chain: B
Molecule details ›
Chain: B
Length: 330 amino acids
Theoretical weight: 37.43 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P48356 (Residues: 328-633; Coverage: 27%)
Gene names: Db, Lepr, Obr
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: R32
Unit cell:
a: 132.678Å b: 132.678Å c: 249.191Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.223 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

Quick links

Citations

4 citation in other articles

Structural insights into the mechanism of leptin receptor activation.
Saxton et al. (2023)
3 more