› 7z42

X-ray diffraction
2.42Å resolution

Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 2B

Released:
DOI: 10.2210/pdb7z42/pdb
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PDB entry 7z42 coloured by chain, front view.
PDB entry 7z42 coloured by chain, side view.
PDB entry 7z42 coloured by chain, top view.
Source organisms:
Primary publication:
Type B and type A influenza polymerases have evolved distinct binding interfaces to recruit the RNA polymerase II CTD.
Krischuns T, Isel C, Drncova P, Lukarska M, Pflug A, Paisant S, Navratil V, Cusack S, Naffakh N
OpenAccess logo PLoS Pathog 18 e1010328 (2022)
PMID: 35605026

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-150309 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Polymerase acidic protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 751 amino acids
Theoretical weight: 85.82 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chains: B, E
Molecule details ›
Chains: B, E
Length: 772 amino acids
Theoretical weight: 86.21 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chains: C, F
DNA-directed RNA polymerase II subunit RPB1 Chains: G, I, X, Y
RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*A)-3') Chains: H, V

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 16 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 130.93Å b: 202.269Å c: 135.733Å
α: 90° β: 110.538° γ: 90°
R-values:
R R work R free
0.225 0.223 0.257
Expression systems:
  • Trichoplusia ni
  • Not provided

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Citations

7 citation in other articles

An intermediate state allows influenza polymerase to switch smoothly between transcription and replication cycles.
Li et al. (2023)
6 more

1 mention without citation

Type B and type A influenza polymerases have evolved distinct binding interfaces to recruit the RNA polymerase II CTD.
Krischuns et al. (2022)

PDB-REDO

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