› 7z43

X-ray diffraction
3.12Å resolution

Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 1B and 2B

Released:
DOI: 10.2210/pdb7z43/pdb
PDB entry 7z43 coloured by chain, front view.
PDB entry 7z43 coloured by chain, side view.
PDB entry 7z43 coloured by chain, top view.
Source organisms:
Primary publication:
Type B and type A influenza polymerases have evolved distinct binding interfaces to recruit the RNA polymerase II CTD.
Krischuns T, Isel C, Drncova P, Lukarska M, Pflug A, Paisant S, Navratil V, Cusack S, Naffakh N
OpenAccess logo PLoS Pathog 18 e1010328 (2022)
PMID: 35605026

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-169171 (preferred)
Entry contents:
4 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (7 distinct):
Polymerase acidic protein Chain: AAA
Molecule details ›
Chain: AAA
Length: 751 amino acids
Theoretical weight: 85.82 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: BBB
Molecule details ›
Chain: BBB
Length: 772 amino acids
Theoretical weight: 86.21 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: CCC
Molecule details ›
Chain: CCC
Length: 798 amino acids
Theoretical weight: 90.84 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8X3 (Residues: 1-770; Coverage: 100%)
Gene name: PB2
Sequence domains:
SER-TYR-SER-PRO-THR-SEP-PRO Chains: XXX, YYY
Molecule details ›
Chains: XXX, YYY
Length: 28 amino acids
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*CP*CP*UP*GP*CP*U)-3') Chain: RRR
Molecule details ›
Chain: RRR
Length: 18 nucleotides
Theoretical weight: 5.58 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*G)-3') Chain: VVV
Molecule details ›
Chain: VVV
Length: 14 nucleotides
Theoretical weight: 4.56 KDa
RNA (5'-R(P*AP*AP*UP*CP*A)-3') Chain: MaM
Molecule details ›
Chain: MaM
Length: 13 nucleotides
Theoretical weight: 4.11 KDa

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand IC5 1 x IC5
1 modified residue:
Ligand SEP 8 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P3221
Unit cell:
a: 200.819Å b: 200.819Å c: 257.018Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.24 0.272
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

2 citation in other articles

An intermediate state allows influenza polymerase to switch smoothly between transcription and replication cycles.
Li et al. (2023)
1 more

1 mention without citation

Type B and type A influenza polymerases have evolved distinct binding interfaces to recruit the RNA polymerase II CTD.
Krischuns et al. (2022)