› 7z5q

X-ray diffraction
1.8Å resolution

Crystal structure of human insulin, crystallised in the presence of macrophage migration inhibitory factor (MIF) and p-Hydroxyphenylpyruvate (HPP)

Released:
DOI: 10.2210/pdb7z5q/pdb
PDB entry 7z5q coloured by chain, front view.
PDB entry 7z5q coloured by chain, side view.
PDB entry 7z5q coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: van den Elsen JMH, Wahid AA, Crennell SJ

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I213
Unit cell:
a: 77.939Å b: 77.939Å c: 77.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.183
Expression system: Escherichia coli

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