› 7zc6

Electron Microscopy
4.27Å resolution

Na+ - translocating ferredoxin: NAD+ reductase (Rnf) of C. tetanomorphum

Released:
DOI: 10.2210/pdb7zc6/pdb
PDB entry 7zc6 coloured by chain, front view.
PDB entry 7zc6 coloured by chain, side view.
PDB entry 7zc6 coloured by chain, top view.
Source organism: Clostridium tetanomorphum
Primary publication:
Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum.
Vitt S, Prinz S, Eisinger M, Ermler U, Buckel W
OpenAccess logo Nat Commun 13 6315 (2022)
PMID: 36274063
Related structures: EMD-14622

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106036 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Ion-translocating oxidoreductase complex subunit A Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 20.59 KDa
Source organism: Clostridium tetanomorphum
Expression system: Clostridium tetanomorphum
UniProt:
  • Canonical: A0A923J0P9 (Residues: 1-191; Coverage: 100%)
Gene names: HGG79_01590, rnfA, rsxA
Sequence domains: Rnf-Nqr subunit, membrane protein
Ion-translocating oxidoreductase complex subunit B Chain: B
Molecule details ›
Chain: B
Length: 274 amino acids
Theoretical weight: 28.01 KDa
Source organism: Clostridium tetanomorphum
Expression system: Clostridium tetanomorphum
UniProt:
  • Canonical: A0A923E755 (Residues: 1-274; Coverage: 100%)
Gene names: HGG79_01585, rnfB
Sequence domains:
Ion-translocating oxidoreductase complex subunit C Chain: C
Molecule details ›
Chain: C
Length: 435 amino acids
Theoretical weight: 47.16 KDa
Source organism: Clostridium tetanomorphum
Expression system: Clostridium tetanomorphum
UniProt:
  • Canonical: A0A923IZ11 (Residues: 1-435; Coverage: 100%)
Gene names: HGG79_01610, rnfC, rsxC
Sequence domains:
Ion-translocating oxidoreductase complex subunit D Chain: D
Molecule details ›
Chain: D
Length: 310 amino acids
Theoretical weight: 32.99 KDa
Source organism: Clostridium tetanomorphum
Expression system: Clostridium tetanomorphum
UniProt:
  • Canonical: A0A923IYK2 (Residues: 1-310; Coverage: 100%)
Gene names: HGG79_01605, rnfD
Sequence domains: NQR2, RnfD, RnfE family
Ion-translocating oxidoreductase complex subunit E Chain: E
Molecule details ›
Chain: E
Length: 201 amino acids
Theoretical weight: 21.21 KDa
Source organism: Clostridium tetanomorphum
Expression system: Clostridium tetanomorphum
UniProt:
  • Canonical: A0A923IZB8 (Residues: 2-202; Coverage: 100%)
Gene names: HGG79_01595, rnfE
Sequence domains: Rnf-Nqr subunit, membrane protein
Ion-translocating oxidoreductase complex subunit G Chain: G
Molecule details ›
Chain: G
Length: 189 amino acids
Theoretical weight: 19.5 KDa
Source organism: Clostridium tetanomorphum
Expression system: Clostridium tetanomorphum
UniProt:
  • Canonical: A0A923J084 (Residues: 1-189; Coverage: 100%)
Gene names: HGG79_01600, rnfG
Sequence domains: FMN-binding domain

Ligands and Environments

4 bound ligands:
Ligand FE 1 x FE
Ligand SF4 7 x SF4
Ligand FMN 3 x FMN
Ligand RBF 1 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.27Å
Relevant EMDB volumes: EMD-14622
Expression system: Clostridium tetanomorphum

Quick links

Citations

1 review citation

Mechanisms for Generating Low Potential Electrons across the Metabolic Diversity of Nitrogen-Fixing Bacteria.
Alleman et al. (2023)
6 other articles

1 mention without citation

Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum.
Vitt et al. (2022)