› 7zfw

Electron Microscopy
3.8Å resolution

Structure of human 80S ribosome obtained from ssDNA coated grid

Released:
DOI: 10.2210/pdb7zfw/pdb
PDB entry 7zfw coloured by chain, front view.
PDB entry 7zfw coloured by chain, side view.
PDB entry 7zfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.
Hrebík D, Gondová M, Valentová L, Füzik T, Přidal A, Nováček J, Plevka P
OpenAccess logo Acta Crystallogr D Struct Biol 78 1337-1346 (2022)
PMID: 36322417
Related structures: EMD-14704

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197366 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (615-MER) Chain: L5
Molecule details ›
Chain: L5
Length: 3774 nucleotides
Theoretical weight: 1222.87 KDa
Source organism: Homo sapiens
Sequence domains: Eukaryotic large subunit ribosomal RNA

Ligands and Environments

No bound ligands
3 modified residues:
Ligand OMG 1 x OMG
Ligand A2M 1 x A2M
Ligand 2MG 1 x 2MG

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-14704

Quick links

Citations

1 citations in other articles

Cryo-EM sample preparation for high-resolution structure studies.
Wang et al. (2024)

1 mention without citation

Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.
Hrebík et al. (2022)