› 7zg9

X-ray diffraction
1.76Å resolution

Structure of yeast Sec14p with himbacine

Released:
DOI: 10.2210/pdb7zg9/pdb
PDB entry 7zg9 coloured by chain, front view.
PDB entry 7zg9 coloured by chain, side view.
PDB entry 7zg9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Mechanisms by Which Small Molecules of Diverse Chemotypes Arrest Sec14 Lipid Transfer Activity.
Chen XR, Poudel L, Hong Z, Johnen P, Katti S, Tripathi A, Nile AH, Green SM, Khan D, Schaaf G, Bono F, Bankaitis VA, Igumenova TI
J Biol Chem 102861 (2023)
PMID: 36603766

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SEC14 cytosolic factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 33.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P24280 (Residues: 3-301; Coverage: 97%)
Gene names: PIT1, SEC14, YM9582.04, YMR079W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand KO0 2 x KO0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 86.593Å b: 86.593Å c: 148.746Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.222
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Mechanisms by which small molecules of diverse chemotypes arrest Sec14 lipid transfer activity.
Chen et al. (2023)