7zhg

Electron Microscopy
2.25Å resolution

High-resolution cryo-EM structure of Pyrococcus abyssi 30S ribosomal subunit bound to mRNA and initiator tRNA anticodon stem-loop

Released:
Source organism: Pyrococcus abyssi GE5
Related structures: EMD-14731

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
PDBe Complex ID:
PDB-CPX-124655 (preferred)
Entry contents:
28 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (31 distinct):
Small ribosomal subunit protein eS1 Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.89 KDa
Source organism: Pyrococcus abyssi GE5
UniProt:
  • Canonical: Q9V2K7 (Residues: 1-199; Coverage: 100%)
Gene names: PAB0035, PYRAB00680, rps3ae
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 202 amino acids
Theoretical weight: 23.03 KDa
Source organism: Pyrococcus abyssi GE5
UniProt:
  • Canonical: Q9V191 (Residues: 1-202; Coverage: 100%)
Gene names: PAB0368, PYRAB05370, rps2
Sequence domains: Ribosomal protein S2
Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein Chain: C
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein eS4 Chain: E
Small ribosomal subunit protein uS5 Chain: F
Small ribosomal subunit protein eS6 Chain: G
Small ribosomal subunit protein uS7 Chain: H
Small ribosomal subunit protein uS8 Chain: I
Small ribosomal subunit protein eS8 Chain: J
Small ribosomal subunit protein uS9 Chain: K
Small ribosomal subunit protein uS10 Chain: L
Small ribosomal subunit protein uS11 Chain: M
Small ribosomal subunit protein uS12 Chain: N
Small ribosomal subunit protein uS13 Chain: O
Small ribosomal subunit protein uS14 Chain: P
Small ribosomal subunit protein uS15 Chain: Q
Small ribosomal subunit protein uS17 Chain: R
Small ribosomal subunit protein eS17 Chain: S
Small ribosomal subunit protein uS19 Chain: T
Small ribosomal subunit protein eS19 Chain: U
Small ribosomal subunit protein eS24 Chain: V
Small ribosomal subunit protein eS27 Chain: W
Small ribosomal subunit protein eS28 Chain: X
Small ribosomal subunit protein eS31 Chain: Y
Small ribosomal subunit protein uS3 Chain: Z
Small ribosomal subunit protein eS32 Chain: 0
Large ribosomal subunit protein eL8 Chain: 3
rRNA 16S Chain: 2
Messenger RNA Chain: 5
Met-tRNAiMet Chain: 4

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.25Å
Relevant EMDB volumes: EMD-14731