7zit

X-ray diffraction
1.79Å resolution

14-3-3 in complex with SARS-COV2 N phospho-peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Nucleoprotein Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 799 Da
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Not provided
UniProt:
  • Canonical: P0DTC9 (Residues: 194-200; Coverage: 2%)
Gene name: N

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 72.18Å b: 83.517Å c: 111.356Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.205
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided