7zju

X-ray diffraction
2.17Å resolution

Structure of human USPL1 in covalent complex with SUMO3-2Br probe

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-229970 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SUMO-specific isopeptidase USPL1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 287 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5W0Q7 (Residues: 218-502; Coverage: 26%)
Gene names: C13orf22, D13S106, USPL1
Sequence domains: Ubiquitin-specific peptidase-like, SUMO isopeptidase
Small ubiquitin-related modifier 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 90 amino acids
Theoretical weight: 10.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55854 (Residues: 2-91; Coverage: 87%)
Gene names: SMT3A, SMT3H1, SUMO3
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 66.06Å b: 84.833Å c: 71.106Å
α: 90° β: 92.257° γ: 90°
R-values:
R R work R free
0.194 0.192 0.238
Expression system: Escherichia coli