› 7zka

Electron Microscopy
2.9Å resolution

ABCB1 V978C mutant (mABCB1) in the outward facing state bound to AAC

Released:
DOI: 10.2210/pdb7zka/pdb
PDB entry 7zka coloured by chain, front view.
PDB entry 7zka coloured by chain, side view.
PDB entry 7zka coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Tracing the substrate translocation mechanism in P-glycoprotein.
Gewering T, Waghray D, Parey K, Jung H, Tran NNB, Zapata J, Zhao P, Chen H, Januliene D, Hummer G, Urbatsch I, Moeller A, Zhang Q
Elife 12 (2024)
PMID: 38259172
Related structures: EMD-14760

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent translocase ABCB1 Chain: A
Molecule details ›
Chain: A
Length: 1328 amino acids
Theoretical weight: 146.08 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P21447 (Residues: 1-1276; Coverage: 100%)
Gene names: Abcb1a, Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand JIZ 1 x JIZ
Ligand ATP 2 x ATP
Ligand MG 2 x MG
Ligand LMN 1 x LMN
Ligand Y01 3 x Y01
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-14760
Expression system: Komagataella pastoris

Quick links

Citations

4 citation in other articles

The transport activity of the multidrug ABC transporter BmrA does not require a wide separation of the nucleotide-binding domains.
Di Cesare et al. (2024)
3 more