› 7zlh

Electron Microscopy
2.75Å resolution

Cryo-EM structure of C-mannosyltransferase CeDPY19, in apo state, bound to CMT2-Fab and anti-Fab nanobody

Released:
DOI: 10.2210/pdb7zlh/pdb
PDB entry 7zlh coloured by chain, front view.
PDB entry 7zlh coloured by chain, side view.
PDB entry 7zlh coloured by chain, top view.
Source organisms:
Primary publication:
Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase.
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
OpenAccess logo Nat Chem Biol (2023)
PMID: 36604564
Related structures: EMD-14780

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273583 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CMT2-Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Anti-Fab nanobody Chain: K
Molecule details ›
Chain: K
Length: 123 amino acids
Theoretical weight: 13.39 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
CMT2-Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.24 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
C-mannosyltransferase dpy-19 Chain: A
Molecule details ›
Chain: A
Length: 707 amino acids
Theoretical weight: 80.89 KDa
Source organism: Caenorhabditis elegans
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34413 (Residues: 1-683; Coverage: 100%)
Gene names: F22B7.10, dpy-19
Sequence domains: Q-cell neuroblast polarisation

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.75Å
Relevant EMDB volumes: EMD-14780
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

A global view of the human post-translational modification landscape.
Kitamura et al. (2023)
3 more