› 7zlu

X-ray diffraction
2.05Å resolution

Catalytic domain of UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum in complex with UDP-2-deoxy-2-fluoro-D-glucose

Released:
DOI: 10.2210/pdb7zlu/pdb
PDB entry 7zlu coloured by chain, front view.
PDB entry 7zlu coloured by chain, side view.
PDB entry 7zlu coloured by chain, top view.
Source organism: Chaetomium thermophilum
Primary publication:
A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpoint.
Guay KP, Ibba R, Kiappes JL, Vasiljević S, Bonì F, De Benedictis M, Zeni I, Le Cornu JD, Hensen M, Chandran AV, Kantsadi AL, Caputo AT, Blanco Capurro JI, Bayo Y, Hill JC, Hudson K, Lia A, Brun J, Withers SG, Martí M, Biasini E, Santino A, De Rosa M, Milani M, Modenutti CP, Hebert DN, Zitzmann N, Roversi P
OpenAccess logo iScience 26 107919 (2023)
PMID: 37822503

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose-glycoprotein glucosyltransferase-like protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 297 amino acids
Theoretical weight: 35.21 KDa
Source organism: Chaetomium thermophilum
Expression system: Homo sapiens
UniProt:
  • Canonical: G0SB58 (Residues: 1187-1473; Coverage: 19%)
Gene name: CTHT_0048990
Sequence domains: Glucosyltransferase 24

Ligands and Environments

4 bound ligands:
Ligand NAG 3 x NAG
Ligand U2F 3 x U2F
Ligand CA 3 x CA
Ligand PDO 3 x PDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 68.159Å b: 72.316Å c: 72.393Å
α: 110.48° β: 108.33° γ: 108.26°
R-values:
R R work R free
0.228 0.226 0.27
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Rescue of secretion of rare-disease-associated misfolded mutant glycoproteins in UGGT1 knock-out mammalian cells.
Tax et al. (2024)

2 mentions without citation

A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpoint.
Guay et al. (2023)
1 more