› 7zmo

X-ray diffraction
3.75Å resolution

Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G3-052

Released:
DOI: 10.2210/pdb7zmo/pdb
Model geometry
Fit model/data
PDB entry 7zmo coloured by chain, front view.
PDB entry 7zmo coloured by chain, side view.
PDB entry 7zmo coloured by chain, top view.
Source organisms:
Entry authors: Ye M, Makola M, Newman JA, Fairhead M, MacLean E, Krojer T, Aitkenhead H, Bountra C, Gileadi O, von Delft F

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271890 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent DNA helicase Q5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 49.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94762 (Residues: 11-453; Coverage: 45%)
Gene names: RECQ5, RECQL5
Sequence domains:
Gluebody G3-052 Chains: C, K
Molecule details ›
Chains: C, K
Length: 127 amino acids
Theoretical weight: 13.7 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Unit cell:
a: 89.517Å b: 150.87Å c: 269.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.284 0.283 0.306
Expression system: Escherichia coli

Quick links

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO