› 7zmr

X-ray diffraction
3.3Å resolution

Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G2*-011

Released:
DOI: 10.2210/pdb7zmr/pdb
PDB entry 7zmr coloured by chain, front view.
PDB entry 7zmr coloured by chain, side view.
PDB entry 7zmr coloured by chain, top view.
Source organisms:
Entry authors: Ye M, Makola M, Newman JA, Fairhead M, MacLean E, Krojer T, Aitkenhead H, Bountra C, Gileadi O, von Delft F

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271893 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent DNA helicase Q5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 49.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94762 (Residues: 11-453; Coverage: 45%)
Gene names: RECQ5, RECQL5
Sequence domains:
Gluebody G2*-011 Chains: C, K
Molecule details ›
Chains: C, K
Length: 127 amino acids
Theoretical weight: 13.76 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 114.89Å b: 198.954Å c: 172.926Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.292
Expression system: Escherichia coli

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