› 7zo8

Electron Microscopy
3.6Å resolution

cryo-EM structure of CGT ABC transporter in nanodisc apo state

Released:
DOI: 10.2210/pdb7zo8/pdb
PDB entry 7zo8 coloured by chain, front view.
PDB entry 7zo8 coloured by chain, side view.
PDB entry 7zo8 coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella.
Sedzicki J, Ni D, Lehmann F, Wu N, Zenobi R, Jung S, Stahlberg H, Dehio C
Nat Struct Mol Biol 29 1170-1177 (2022)
PMID: 36456825
Related structures: EMD-14843

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + beta-glucan(Side 1) = ADP + phosphate + beta-glucan(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-(1-->2)glucan export ATP-binding/permease protein NdvA Chains: A, B
Molecule details ›
Chains: A, B
Length: 599 amino acids
Theoretical weight: 66.03 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YQ73 (Residues: 1-599; Coverage: 100%)
Gene names: BAB1_1017, cgt, ndvA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand PLC 2 x PLC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-14843
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Immunomodulatory Effect and Biological Significance of β-Glucans.
Zhong et al. (2023)
1 more

1 mention without citation

Cryo-EM structure and B-factor refinement with ensemble representation.
Beton et al. (2024)