› 7zow

X-ray diffraction
1.6Å resolution

Crystal structure of Synechocystis halorhodopsin (SyHR), Cl-pumping mode, O state

Released:
DOI: 10.2210/pdb7zow/pdb
PDB entry 7zow coloured by chain, front view.
PDB entry 7zow coloured by chain, side view.
PDB entry 7zow coloured by chain, top view.
Source organism: Synechocystis sp. PCC 7509
Primary publication:
Structural insights into light-driven anion pumping in cyanobacteria.
Astashkin R, Kovalev K, Bukhdruker S, Vaganova S, Kuzmin A, Alekseev A, Balandin T, Zabelskii D, Gushchin I, Royant A, Volkov D, Bourenkov G, Koonin E, Engelhard M, Bamberg E, Gordeliy V
OpenAccess logo Nat Commun 13 6460 (2022)
PMID: 36309497

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synechocystis halorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.46 KDa
Source organism: Synechocystis sp. PCC 7509
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand LFA 11 x LFA
Ligand OLA 4 x OLA
Ligand OLC 3 x OLC
2 modified residues:
Ligand FME 1 x FME
Ligand LYR 1 x LYR

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P321
Unit cell:
a: 62.406Å b: 62.406Å c: 110.076Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.232
Expression system: Escherichia coli

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