› 7zp5

X-ray diffraction
1.54Å resolution

Crystal structure of designed photoenzyme EnT1.0

Released:
DOI: 10.2210/pdb7zp5/pdb
PDB entry 7zp5 coloured by chain, front view.
PDB entry 7zp5 coloured by chain, side view.
PDB entry 7zp5 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
A Designed Photoenzyme for Enantioselective [2+2]-Cycloadditions.
Trimble JS, Crawshaw R, Hardy FJ, Levy CW, Brown MJB, Fuerst DE, Heyes DJ, Obexer R, Green AP
Nature (2022)
PMID: 36130727

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diisopropyl-fluorophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.24 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SIG4 (Residues: 1-314; Coverage: 100%)
Sequence domains: SMP-30/Gluconolactonase/LRE-like region

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand PBF 2 x PBF

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 173.847Å b: 43.397Å c: 81.764Å
α: 90° β: 93.98° γ: 90°
R-values:
R R work R free
0.197 0.196 0.223
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Intelligent Protein Design and Molecular Characterization Techniques: A Comprehensive Review.
Wang et al. (2023)
1 more