› 7zqu

X-ray diffraction
1.75Å resolution

A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - C72A mutant

Released:
DOI: 10.2210/pdb7zqu/pdb
PDB entry 7zqu coloured by chain, front view.
PDB entry 7zqu coloured by chain, side view.
PDB entry 7zqu coloured by chain, top view.
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Entry authors: Batra-Safferling R, Granzin J

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative blue-light photoreceptor Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.83 KDa
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A8LP63 (Residues: 2-139; Coverage: 99%)
Gene name: Dshi_2006
Sequence domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 91.454Å b: 28.21Å c: 48.018Å
α: 90° β: 119.397° γ: 90°
R-values:
R R work R free
0.189 0.188 0.205
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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