7zra

X-ray diffraction
2.8Å resolution

Crystal structure of E.coli LexA in complex with nanobody NbSOS1(Nb14497)

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
Hydrolysis of Ala(84)-|-Gly bond in repressor LexA.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-270079 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LexA repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 202 amino acids
Theoretical weight: 22.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
Nanobody NbSOS1 (Nb14497) Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 132 amino acids
Theoretical weight: 14.6 KDa
Source organism: Lama glama
Expression system: Escherichia coli W

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 59.918Å b: 110.75Å c: 231.796Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.259
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli W