› 7zsl

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 289 amino acids
Theoretical weight: 32.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 1-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily

Ligands and Environments

4 bound ligands:
Ligand JTO 6 x JTO
Ligand EDO 3 x EDO
Ligand SO4 10 x SO4
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 79.12Å b: 185.1Å c: 79.18Å
α: 90° β: 119.94° γ: 90°
R-values:
R R work R free
0.226 0.225 0.268
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Design, Synthesis, Biological Evaluation, and Crystallographic Study of Novel Purine Nucleoside Phosphorylase Inhibitors.
Skácel et al. (2023)