7zuw

Electron Microscopy
4.3Å resolution

Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 82-mer (preferred)
PDBe Complex ID:
PDB-CPX-271532 (preferred)
Entry contents:
77 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (82 distinct):
Small ribosomal subunit protein uS2A Chain: AA
Molecule details ›
Chain: AA
Length: 206 amino acids
Theoretical weight: 22.81 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32905 (Residues: 2-207; Coverage: 82%)
Gene names: NAB1, NAB1A, RPS0A, YGR214W, YST1
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein eS1A Chain: AB
Molecule details ›
Chain: AB
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33442 (Residues: 1-255; Coverage: 100%)
Gene names: L9753.9, PLC1, RPS10A, RPS1A, YLR441C
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS5 Chain: AC
Small ribosomal subunit protein uS3 Chain: AD
Small ribosomal subunit protein eS4A Chain: AE
Small ribosomal subunit protein uS7 Chain: AF
Small ribosomal subunit protein eS6A Chain: AG
Small ribosomal subunit protein eS7A Chain: AH
Small ribosomal subunit protein eS8B Chain: AI
Small ribosomal subunit protein uS4A Chain: AJ
Small ribosomal subunit protein eS10A Chain: AK
Small ribosomal subunit protein uS17A Chain: AL
Small ribosomal subunit protein eS12 Chain: AM
Small ribosomal subunit protein uS15 Chain: AN
Small ribosomal subunit protein uS11B Chain: AO
Small ribosomal subunit protein uS19 Chain: AP
Small ribosomal subunit protein uS9A Chain: AQ
Small ribosomal subunit protein eS17A Chain: AR
Small ribosomal subunit protein uS13A Chain: AS
Small ribosomal subunit protein eS19A Chain: AT
Small ribosomal subunit protein uS10 Chain: AU
Small ribosomal subunit protein eS21A Chain: AV
Small ribosomal subunit protein uS8A Chain: AW
Small ribosomal subunit protein uS12A Chain: AX
Small ribosomal subunit protein eS24A Chain: AY
Small ribosomal subunit protein eS25A Chain: AZ
Small ribosomal subunit protein eS26A Chain: Aa
Small ribosomal subunit protein eS27A Chain: Ab
Small ribosomal subunit protein eS28A Chain: Ac
Small ribosomal subunit protein uS14A Chain: Ad
Small ribosomal subunit protein eS30A Chain: Ae
Small ribosomal subunit protein eS31 Chain: Af
Small ribosomal subunit protein RACK1 Chain: Ag
Large ribosomal subunit protein uL2A Chain: BA
Large ribosomal subunit protein uL3 Chain: BB
Large ribosomal subunit protein uL4A Chain: BC
Large ribosomal subunit protein uL18 Chain: BD
Large ribosomal subunit protein eL6B Chain: BE
Large ribosomal subunit protein uL30A Chain: BF
Large ribosomal subunit protein eL8A Chain: BG
Large ribosomal subunit protein uL6A Chain: BH
Large ribosomal subunit protein uL16 Chain: BI
Large ribosomal subunit protein uL5A Chain: BJ
Large ribosomal subunit protein eL13A Chain: BK
Large ribosomal subunit protein eL14A Chain: BL
Large ribosomal subunit protein eL15A Chain: BM
Large ribosomal subunit protein uL13A Chain: BN
Large ribosomal subunit protein uL22A Chain: BO
Large ribosomal subunit protein eL18A Chain: BP
Large ribosomal subunit protein eL19A Chain: BQ
Large ribosomal subunit protein eL20A Chain: BR
Large ribosomal subunit protein eL21A Chain: BS
Large ribosomal subunit protein eL22A Chain: BT
Large ribosomal subunit protein uL14A Chain: BU
Large ribosomal subunit protein eL24A Chain: BV
Large ribosomal subunit protein uL23 Chain: BW
Large ribosomal subunit protein uL24A Chain: BX
Large ribosomal subunit protein eL27A Chain: BY
Large ribosomal subunit protein uL15 Chain: BZ
Large ribosomal subunit protein eL29 Chain: Ba
Large ribosomal subunit protein eL30 Chain: Bb
Large ribosomal subunit protein eL31A Chain: Bc
Large ribosomal subunit protein eL32 Chain: Bd
Large ribosomal subunit protein eL33A Chain: Be
Large ribosomal subunit protein eL34A Chain: Bf
Large ribosomal subunit protein uL29A Chain: Bg
Large ribosomal subunit protein eL36A Chain: Bh
Large ribosomal subunit protein eL37A Chain: Bi
Large ribosomal subunit protein eL38 Chain: Bj
Large ribosomal subunit protein eL39 Chain: Bk
Large ribosomal subunit protein eL40A Chain: Bl
Small ribosomal subunit protein eS32A Chain: Bm
Large ribosomal subunit protein eL42A Chain: Bn
Large ribosomal subunit protein eL43A Chain: Bo
RQC trigger complex helicase SLH1 Chain: CA
RQC trigger complex subunit CUE3 Chain: CB
RQC trigger complex subunit RQT4 Chain: CC
18S Ribosomal RNA Chain: 2
5.8S ribosomal RNA Chain: 3
5S ribosomal RNA Chain: 4
25S ribosomal RNA Chain: 5
tRNA Chain: 6

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.3Å
Relevant EMDB volumes: EMD-14978
Expression system: Saccharomyces cerevisiae