› 7zyi

Electron Microscopy
2.88Å resolution

Structure of the human sodium/bile acid cotransporter (NTCP) in complex with Fab and nanobody

Released:
DOI: 10.2210/pdb7zyi/pdb
PDB entry 7zyi coloured by chain, front view.
PDB entry 7zyi coloured by chain, side view.
PDB entry 7zyi coloured by chain, top view.
Source organisms:
Primary publication:
Structure of human NTCP reveals the basis of recognition and sodium-driven transport of bile salts into the liver.
Liu H, Irobalieva RN, Bang-Sørensen R, Nosol K, Mukherjee S, Agrawal P, Stieger B, Kossiakoff AA, Locher KP
OpenAccess logo Cell Res (2022)
PMID: 35726088
Related structures: EMD-15024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274555 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hepatic sodium/bile acid cotransporter Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 38.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14973 (Residues: 1-349; Coverage: 100%)
Gene names: GIG29, NTCP, SLC10A1
Sequence domains: Sodium Bile acid symporter family
heavy chain of Fab Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
light chain of Fab Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.42 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Nanobody Chain: K
Molecule details ›
Chain: K
Length: 120 amino acids
Theoretical weight: 13.12 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand CLR 2 x CLR
Ligand CHO 2 x CHO
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-15024
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

4 review citations

In vitro cell culture models to study hepatitis B and D virus infection.
Guo et al. (2023)
3 more

4 mentions without citation

Role of the Sodium-Dependent Organic Anion Transporter (SOAT/SLC10A6) in Physiology and Pathophysiology.
Wannowius et al. (2023)
3 more