› 8aa0

Electron Microscopy
3.2Å resolution

Levan utilisation machinery (utilisome) with levan fructo-oligosaccharides DP 8-12

Released:
DOI: 10.2210/pdb8aa0/pdb
PDB entry 8aa0 coloured by chain, front view.
PDB entry 8aa0 coloured by chain, side view.
PDB entry 8aa0 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Outer membrane utilisomes mediate glycan uptake in gut Bacteroidetes.
White JBR, Silale A, Feasey M, Heunis T, Zhu Y, Zheng H, Gajbhiye A, Firbank S, Baslé A, Trost M, Bolam DN, van den Berg B, Ranson NA
Nature (2023)
PMID: 37286596
Related structures: EMD-15289

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-217873 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
beta-fructofuranosidase Chains: E, M
Molecule details ›
Chains: E, M
Length: 523 amino acids
Theoretical weight: 59.3 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Bacteroides thetaiotaomicron VPI-5482
UniProt:
  • Canonical: Q8A6W6 (Residues: 1-523; Coverage: 100%)
Gene name: BT_1760
Sequence domains:
DUF4960 domain-containing protein Chains: G, T
Molecule details ›
Chains: G, T
Length: 461 amino acids
Theoretical weight: 50.43 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Bacteroides thetaiotaomicron VPI-5482
UniProt:
  • Canonical: Q8A6W5 (Residues: 1-461; Coverage: 100%)
Gene name: BT_1761
Sequence domains: Domain of unknown function (DUF4960)
SusD homolog Chains: B, J
Molecule details ›
Chains: B, J
Length: 580 amino acids
Theoretical weight: 66.14 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Bacteroides thetaiotaomicron VPI-5482
UniProt:
  • Canonical: Q8A6W4 (Residues: 1-570; Coverage: 100%)
Gene name: BT_1762
Sequence domains:
SusC homolog Chains: A, I
Molecule details ›
Chains: A, I
Length: 1041 amino acids
Theoretical weight: 115.48 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Bacteroides thetaiotaomicron VPI-5482
UniProt:
  • Canonical: Q8A6W3 (Residues: 1-1041; Coverage: 100%)
Gene name: BT_1763
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FRU
Carbohydrate polymer
1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-15289
Expression system: Bacteroides thetaiotaomicron VPI-5482

Quick links

Citations

1 review citation

Therapeutic Potential of Fungal Polysaccharides in Gut Microbiota Regulation: Implications for Diabetes, Neurodegeneration, and Oncology.
Barcan et al. (2024)
13 other articles