› 8aq4

X-ray diffraction
2.62Å resolution

In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with TITC and lyso-PE

Released:
DOI: 10.2210/pdb8aq4/pdb
PDB entry 8aq4 coloured by chain, front view.
PDB entry 8aq4 coloured by chain, side view.
PDB entry 8aq4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein N-acyltransferase.
Smithers L, Degtjarik O, Weichert D, Huang CY, Boland C, Bowen K, Oluwole A, Lutomski C, Robinson CV, Scanlan EM, Wang M, Olieric V, Shalev-Benami M, Caffrey M
OpenAccess logo Sci Adv 9 eadf5799 (2023)
PMID: 37390210

Function and Biology Details

Reaction catalysed: 
A phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine = a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apolipoprotein N-acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 532 amino acids
Theoretical weight: 59.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23930 (Residues: 1-512; Coverage: 100%)
Gene names: JW0654, b0657, cutE, lnt
Sequence domains:

Ligands and Environments

9 bound ligands:
Ligand PG5 4 x PG5
Ligand GOL 6 x GOL
Ligand 6OU 3 x 6OU
Ligand QGT 1 x QGT
Ligand PG6 1 x PG6
Ligand TRS 1 x TRS
Ligand ACT 1 x ACT
Ligand LMT 3 x LMT
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3221
Unit cell:
a: 160.111Å b: 160.111Å c: 90.934Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.229
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

7.10 MAG. A Novel Host Monoacylglyceride for In Meso (Lipid Cubic Phase) Crystallization of Membrane Proteins.
Krawinski et al. (2024)
2 more

1 mention without citation

Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein N-acyltransferase.
Smithers et al. (2023)