› 8awk

X-ray diffraction
1.58Å resolution

Structure of recombinant human beta-glucocerebrosidase in complex with D-carbaxylosyl chloride

Released:
DOI: 10.2210/pdb8awk/pdb
PDB entry 8awk coloured by chain, front view.
PDB entry 8awk coloured by chain, side view.
PDB entry 8awk coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Rowland RJ, Davies GJ

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal acid glucosylceramidase Chain: AAA
Molecule details ›
Chain: AAA
Length: 497 amino acids
Theoretical weight: 55.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 21 x EDO
Ligand NAG 2 x NAG
Ligand OIW 1 x OIW
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 53.277Å b: 76.465Å c: 68.469Å
α: 90° β: 102.047° γ: 90°
R-values:
R R work R free
0.153 0.152 0.185
Expression system: Trichoplusia ni

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