› 8b81

X-ray diffraction
1.58Å resolution

The structure of Gan1D W433A in complex with cellobiose-6-phosphate

Released:
DOI: 10.2210/pdb8b81/pdb
PDB entry 8b81 coloured by chain, front view.
PDB entry 8b81 coloured by chain, side view.
PDB entry 8b81 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Snyder J, Lansky S, Zehavi A, Shoham Y, Shoham G

Function and Biology Details

Reaction catalysed: 
A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Putative 6-phospho-beta-galactobiosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 55.15 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: W8QF82 (Residues: 1-478; Coverage: 100%)
Gene name: gan1D
Sequence domains: Glycosyl hydrolase family 1

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, BG6
Carbohydrate polymer
1 bound ligand:
Ligand IMD 4 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 107.9Å b: 68.38Å c: 149.8Å
α: 90° β: 99.36° γ: 90°
R-values:
R R work R free
0.17 0.169 0.201
Expression system: Escherichia coli

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