› 8bgo

X-ray diffraction
3.08Å resolution

N,N-diacetylchitobiose deacetylase from Pyrococcus chitonophagus with substrate N,N-diacetylchitobiose

Released:
DOI: 10.2210/pdb8bgo/pdb
PDB entry 8bgo coloured by chain, front view.
PDB entry 8bgo coloured by chain, side view.
PDB entry 8bgo coloured by chain, top view.
Source organism: Thermococcus chitonophagus
Primary publication:
Structural, Thermodynamic and Enzymatic Characterization of N,N-Diacetylchitobiose Deacetylase from Pyrococcus chitonophagus.
Biniek-Antosiak K, Bejger M, Śliwiak J, Baranowski D, Mohammed ASA, Svergun DI, Rypniewski W
OpenAccess logo Int J Mol Sci 23 (2022)
PMID: 36555375

Function and Biology Details

Reaction catalysed: 
N,N'-diacetylchitobiose + H(2)O = beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Diacetylchitobiose deacetylase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 271 amino acids
Theoretical weight: 31.43 KDa
Source organism: Thermococcus chitonophagus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A160VQZ8 (Residues: 1-267; Coverage: 100%)
Gene names: A3L04_02905, CHITON_0574
Sequence domains: GlcNAc-PI de-N-acetylase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 79.164Å b: 151.662Å c: 167.092Å
α: 90° β: 93.33° γ: 90°
R-values:
R R work R free
0.169 0.168 0.215
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structural, Thermodynamic and Enzymatic Characterization of N,N-Diacetylchitobiose Deacetylase from Pyrococcus chitonophagus.
Biniek-Antosiak et al. (2022)