› 8bhh

X-ray diffraction
1.69Å resolution

The crystal structure of a feruloyl esterase C from Fusarium oxysporum in complex with p-coumaric acid

Released:
DOI: 10.2210/pdb8bhh/pdb
PDB entry 8bhh coloured by chain, front view.
PDB entry 8bhh coloured by chain, side view.
PDB entry 8bhh coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Crystal structure of the Fusarium oxysporum tannase-like feruloyl esterase FaeC in complex with p-coumaric acid provides insight into ligand binding.
Ferousi C, Kosinas C, Nikolaivits E, Topakas E, Dimarogona M
FEBS Lett 597 1415-1427 (2023)
PMID: 36961270

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Carboxylic ester hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 508 amino acids
Theoretical weight: 56.14 KDa
Source organism: Fusarium oxysporum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A2H3G048 (Residues: 52-557; Coverage: 94%)
Gene name: AU210_015050
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
7 bound ligands:
Ligand HC4 2 x HC4
Ligand NAG 4 x NAG
Ligand EDO 10 x EDO
Ligand CA 2 x CA
Ligand PG4 1 x PG4
Ligand P6G 1 x P6G
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 67.704Å b: 89.637Å c: 116.978Å
α: 90° β: 103.99° γ: 90°
R-values:
R R work R free
0.173 0.171 0.217
Expression system: Komagataella pastoris

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