› 8cwc

X-ray diffraction
1.48Å resolution

20ns Temperature-Jump (Light) XFEL structure of Lysozyme Bound to N,N'-diacetylchitobiose

Released:
DOI: 10.2210/pdb8cwc/pdb
PDB entry 8cwc coloured by chain, front view.
PDB entry 8cwc coloured by chain, side view.
PDB entry 8cwc coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography.
Wolff AM, Nango E, Young ID, Brewster AS, Kubo M, Nomura T, Sugahara M, Owada S, Barad BA, Ito K, Bhowmick A, Carbajo S, Hino T, Holton JM, Im D, O'Riordan LJ, Tanaka T, Tanaka R, Sierra RG, Yumoto F, Tono K, Iwata S, Sauter NK, Fraser JS, Thompson MC
OpenAccess logo Nat Chem (2023)
PMID: 37723259

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

Carbohydrate polymer : NEW Components: NDG, NAG
Carbohydrate polymer
2 bound ligands:
Ligand NA 2 x NA
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SACLA BEAMLINE BL3
Spacegroup: P43212
Unit cell:
a: 77.061Å b: 77.061Å c: 37.223Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.169
Expression system: Not provided

Quick links

Citations

1 review citation

Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics.
Hekstra DR. (2023)
1 other article