› 8d0o

X-ray diffraction
2.1Å resolution

Human alpha1,3-fucosyltransferase FUT9, heavy atom derivative

Released:
DOI: 10.2210/pdb8d0o/pdb
PDB entry 8d0o coloured by chain, front view.
PDB entry 8d0o coloured by chain, side view.
PDB entry 8d0o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9.
Kadirvelraj R, Boruah BM, Wang S, Chapla D, Huang C, Ramiah A, Hudson KL, Prudden AR, Boons GJ, Withers SG, Wood ZA, Moremen KW
Nat Chem Biol (2023)
PMID: 37202521

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->4)-(alpha-L-fucosyl-(1->3))-N-acetyl-D-glucosaminyl-R
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 37.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y231 (Residues: 39-359; Coverage: 89%)
Gene name: FUT9
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand IOD 14 x IOD
Ligand CS 9 x CS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I213
Unit cell:
a: 127.12Å b: 127.12Å c: 127.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.209
Expression system: Homo sapiens

Quick links

Citations

2 review citations

Medical Relevance, State-of-the-Art and Perspectives of "Sweet Metacode" in Liquid Biopsy Approaches.
Pinkeova et al. (2024)
1 more