8da9

X-ray diffraction
1.35Å resolution

Coevolved affibody-Z domain pair LL2.c3

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-527155 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin G-binding protein A Chains: A, C
Molecule details ›
Chains: A, C
Length: 67 amino acids
Theoretical weight: 7.82 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02976 (Residues: 213-269; Coverage: 12%)
Gene names: SAOUHSC_00069, spa
Sequence domains: B domain
Affibody LL2.FIIV Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.64 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Affibody LL2.FIIV Chain: D
Molecule details ›
Chain: D
Length: 67 amino acids
Theoretical weight: 7.67 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 53.403Å b: 54.259Å c: 68.601Å
α: 90° β: 94.41° γ: 90°
R-values:
R R work R free
0.176 0.175 0.198
Expression system: Escherichia coli