› 8de7

Electron Microscopy
3.27Å resolution

Cryo-EM structure of the zebrafish two pore domain K+ channel TREK1 (K2P2.1) in DDM detergent

Released:
DOI: 10.2210/pdb8de7/pdb
PDB entry 8de7 coloured by chain, front view.
PDB entry 8de7 coloured by chain, side view.
PDB entry 8de7 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1.
Schmidpeter PAM, Petroff JT, Khajoueinejad L, Wague A, Frankfater C, Cheng WWL, Nimigean CM, Riegelhaupt PM
OpenAccess logo Nat Commun 14 1077 (2023)
PMID: 36841877
Related structures: EMD-27386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-514282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35.56 KDa
Source organism: Danio rerio
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1QAI2 (Residues: 14-334; Coverage: 75%)
Gene name: kcnk2a
Sequence domains: Ion channel

Ligands and Environments

2 bound ligands:
Ligand LMT 1 x LMT
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.27Å
Relevant EMDB volumes: EMD-27386
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Mechanosensing by Vascular Endothelium.
Lim et al. (2024)
11 other articles