› 8dlr

Electron Microscopy
2.51Å resolution

Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with Fab 4-8 (focused refinement of NTD and 4-8)

Released:
DOI: 10.2210/pdb8dlr/pdb
PDB entry 8dlr coloured by chain, front view.
PDB entry 8dlr coloured by chain, side view.
PDB entry 8dlr coloured by chain, top view.
Source organisms:
Primary publication:
SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization.
Mannar D, Saville JW, Sun Z, Zhu X, Marti MM, Srivastava SS, Berezuk AM, Zhou S, Tuttle KS, Sobolewski MD, Kim A, Treat BR, Da Silva Castanha PM, Jacobs JL, Barratt-Boyes SM, Mellors JW, Dimitrov DS, Li W, Subramaniam S
OpenAccess logo Nat Commun 13 4696 (2022)
PMID: 35982054
Related structures: EMD-27512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225307 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Spike glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 1288 amino acids
Theoretical weight: 142.49 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
Fab 4-8 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 255 amino acids
Theoretical weight: 27.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab 4-8 light chain Chain: L
Molecule details ›
Chain: L
Length: 232 amino acids
Theoretical weight: 24.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.51Å
Relevant EMDB volumes: EMD-27512
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Microalgae as an Efficient Vehicle for the Production and Targeted Delivery of Therapeutic Glycoproteins against SARS-CoV-2 Variants.
Dehghani et al. (2022)
3 more