› 8dwy

Electron Microscopy
3.18Å resolution

Chikungunya VLP in complex with neutralizing Fab CHK-265 (asymmetric unit)

Released:
DOI: 10.2210/pdb8dwy/pdb
PDB entry 8dwy coloured by chain, front view.
PDB entry 8dwy coloured by chain, side view.
PDB entry 8dwy coloured by chain, top view.
Source organisms:
Primary publication:
A chikungunya virus-like particle vaccine induces broadly neutralizing and protective antibodies against alphaviruses in humans.
Raju S, Adams LJ, Earnest JT, Warfield K, Vang L, Crowe JE, Fremont DH, Diamond MS
Sci Transl Med 15 eade8273 (2023)
PMID: 37196061
Related structures: EMD-27767

Function and Biology Details

Reaction catalysed: 
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-562732 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Spike glycoprotein E1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 439 amino acids
Theoretical weight: 47.35 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 810-1248; Coverage: 35%)
Sequence domains: Alphavirus E1 glycoprotein
Spike glycoprotein E2 Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 419 amino acids
Theoretical weight: 46.86 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 330-748; Coverage: 34%)
Sequence domains: Alphavirus E2 glycoprotein
Capsid protein Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 151 amino acids
Theoretical weight: 16.46 KDa
Source organism: Chikungunya virus strain Senegal 37997
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5XXP3 (Residues: 111-261; Coverage: 12%)
Sequence domains: Alphavirus core protein
CHK-265 heavy chain Chains: H, V, X, Z
Molecule details ›
Chains: H, V, X, Z
Length: 117 amino acids
Theoretical weight: 13.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CHK-265 light chain Chains: L, U, W, Y
Molecule details ›
Chains: L, U, W, Y
Length: 109 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 12 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.18Å
Relevant EMDB volumes: EMD-27767
Expression system: Homo sapiens

Quick links

Citations

2 review citations

Generating prophylactic immunity against arboviruses in vertebrates and invertebrates.
Rawle et al. (2024)
1 more

1 mention without citation

A chikungunya virus-like particle vaccine induces broadly neutralizing and protective antibodies against alphaviruses in humans.
Raju et al. (2023)