8fd0

X-ray diffraction
3.71Å resolution

Crystal structure of bovine rod opsin in complex with a nanobody

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-526833 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Rhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Nanobody Nb2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 126 amino acids
Theoretical weight: 13.85 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3121
Unit cell:
a: 120.065Å b: 120.065Å c: 230.943Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.292 0.292 0.295
Expression system: Escherichia coli