› 8g18

X-ray diffraction
2.85Å resolution

Heterodimer of the GluN1b-GluN2B NMDA receptor amino-terminal domains bound to allosteric inhibitor 93-108

Released:
DOI: 10.2210/pdb8g18/pdb
PDB entry 8g18 coloured by chain, front view.
PDB entry 8g18 coloured by chain, side view.
PDB entry 8g18 coloured by chain, top view.
Source organisms:
Primary publication:
Novel GluN2B-Selective NMDA Receptor Negative Allosteric Modulator Possesses Intrinsic Analgesic Properties and Enhances Analgesia of Morphine in a Rodent Tail Flick Pain Model.
Harris LD, Regan MC, Myers SJ, Nocilla KA, Akins NS, Tahirovic YA, Wilson LJ, Dingledine R, Furukawa H, Traynelis SF, Liotta DC
OpenAccess logo ACS Chem Neurosci (2023)
PMID: 36779874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-498867 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamate receptor Chains: A, C
Molecule details ›
Chains: A, C
Length: 385 amino acids
Theoretical weight: 42.3 KDa
Source organism: Xenopus laevis
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A8J0TPH2 (Residues: 23-185, 211-407; Coverage: 39%)
Gene names: LOC108699855, grin1.S
Sequence domains: Receptor family ligand binding region
Glutamate receptor ionotropic, NMDA 2B Chains: B, D
Molecule details ›
Chains: B, D
Length: 363 amino acids
Theoretical weight: 41.28 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q00960 (Residues: 32-394; Coverage: 25%)
Gene name: Grin2b
Sequence domains: Receptor family ligand binding region

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 7 x NAG
Ligand NA 2 x NA
Ligand YGW 2 x YGW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: C2
Unit cell:
a: 269.172Å b: 60.076Å c: 145.572Å
α: 90° β: 117.02° γ: 90°
R-values:
R R work R free
0.178 0.174 0.246
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

Glycine Transporter 1 Inhibitors: Predictions on Their Possible Mechanisms in the Development of Opioid Analgesic Tolerance.
Galambos et al. (2024)
1 more

4 mentions without citation

Antiviral Functions of Monoclonal Antibodies against Chikungunya Virus.
Jin et al. (2019)
3 more