› 8g5x

X-ray diffraction
2.2Å resolution

Structure of the Class II Fructose-1,6-Bisphophatase from Francisella tularensis complexed with native metal cofactor Mn++ and substrate Fructose-1,6-Bisphosphate

Released:
DOI: 10.2210/pdb8g5x/pdb
PDB entry 8g5x coloured by chain, front view.
PDB entry 8g5x coloured by chain, side view.
PDB entry 8g5x coloured by chain, top view.
Source organism: Francisella cf. tularensis subsp. novicida 3523
Primary publication:
New structures of Class II Fructose-1,6-Bisphosphatase from Francisella tularensis provide a framework for a novel catalytic mechanism for the entire class.
Selezneva AI, Harding LNM, Gutka HJ, Movahedzadeh F, Abad-Zapatero C
OpenAccess logo PLoS One 18 e0274723 (2023)
PMID: 37352301

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 348 amino acids
Theoretical weight: 37.03 KDa
Source organism: Francisella cf. tularensis subsp. novicida 3523
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NEJ8 (Residues: 1-328; Coverage: 100%)
Gene names: FTT_1631c, gplX
Sequence domains: Bacterial fructose-1,6-bisphosphatase, glpX-encoded

Ligands and Environments

3 bound ligands:
Ligand GOL 13 x GOL
Ligand MN 4 x MN
Ligand FBP 1 x FBP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 63.76Å b: 74.577Å c: 77.191Å
α: 67.893° β: 67.888° γ: 77.014°
R-values:
R R work R free
0.168 0.165 0.227
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

New structures of Class II Fructose-1,6-Bisphosphatase from Francisella tularensis provide a framework for a novel catalytic mechanism for the entire class.
Selezneva et al. (2023)