8gb5

X-ray diffraction
3.35Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-272909 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
Spike protein S1 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 205 amino acids
Theoretical weight: 23.1 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0DTC2 (Residues: 333-530; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
25F9 Heavy chain Chains: B, F, I, L
Molecule details ›
Chains: B, F, I, L
Length: 231 amino acids
Theoretical weight: 24.79 KDa
Source organism: Macaca mulatta
Expression system: Mus musculus
25F9 Light chain Chains: C, E, H, K
Molecule details ›
Chains: C, E, H, K
Length: 216 amino acids
Theoretical weight: 22.77 KDa
Source organism: Macaca mulatta
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 92.485Å b: 105.824Å c: 118.179Å
α: 83.08° β: 67.47° γ: 64.04°
R-values:
R R work R free
0.277 0.275 0.32
Expression systems:
  • Trichoplusia ni
  • Mus musculus