› 8h2v

X-ray diffraction
1.68Å resolution

Cellodextrin phosphorylase from Clostridium thermocellum mutant - all cysteine residues were substituted with serines

Released:
DOI: 10.2210/pdb8h2v/pdb
PDB entry 8h2v coloured by chain, front view.
PDB entry 8h2v coloured by chain, side view.
PDB entry 8h2v coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Entry authors: Kuga T, Sunagawa N, Igarashi K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellodextrin phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 993 amino acids
Theoretical weight: 112.74 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93HT8 (Residues: 2-984; Coverage: 100%)
Gene name: cdp-ym4
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand PO4 2 x PO4
Ligand BGC 2 x BGC
Ligand GOL 15 x GOL
Ligand ACT 11 x ACT
Ligand PEG 3 x PEG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 82.277Å b: 88.52Å c: 88.54Å
α: 98.41° β: 110.69° γ: 110.65°
R-values:
R R work R free
0.185 0.184 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links