› 8ha1

X-ray diffraction
3.5Å resolution

Crystal structure of voltage-gated sodium channel NavAb N49K/L176G mutant in sodium ion condition

Released:
DOI: 10.2210/pdb8ha1/pdb
PDB entry 8ha1 coloured by chain, front view.
PDB entry 8ha1 coloured by chain, side view.
PDB entry 8ha1 coloured by chain, top view.
Source organism: Aliarcobacter butzleri
Primary publication:
The structural basis of divalent cation block in a tetrameric prokaryotic sodium channel.
Irie K, Oda Y, Sumikama T, Oshima A, Fujiyoshi Y
Nat Commun 14 4236 (2023)
PMID: 37454189

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.34 KDa
Source organism: Aliarcobacter butzleri
Expression system: Escherichia coli
UniProt:
  • Canonical: A8EVM5 (Residues: 1-267; Coverage: 100%)
Gene name: Abu_1752
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand LMT 2 x LMT
Ligand 1N7 1 x 1N7
Ligand PX4 8 x PX4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: I422
Unit cell:
a: 126.91Å b: 126.91Å c: 201.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.292 0.291 0.297
Expression system: Escherichia coli

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