› 8hfs

Electron Microscopy
2.98Å resolution

The structure of LcnA, LciA, and the man-PTS of Lactococcus lactis

Released:
DOI: 10.2210/pdb8hfs/pdb
PDB entry 8hfs coloured by chain, front view.
PDB entry 8hfs coloured by chain, side view.
PDB entry 8hfs coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of the Mechanisms of Action and Immunity of Lactococcin A, a Class IId Bacteriocin.
Li R, Duan J, Zhou Y, Wang J
Appl Environ Microbiol e0006623 (2023)
PMID: 36840592
Related structures: EMD-34726

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-531591 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Mannose-specific PTS system, IIC component Chains: C, E, Y
Molecule details ›
Chains: C, E, Y
Length: 270 amino acids
Theoretical weight: 27.57 KDa
Source organism: Lactococcus lactis subsp. lactis KF147
Expression system: Escherichia coli
Mannose-specific PTS system, IID component Chains: D, F, Z
Molecule details ›
Chains: D, F, Z
Length: 307 amino acids
Theoretical weight: 33.63 KDa
Source organism: Lactococcus lactis subsp. lactis KF147
Expression system: Escherichia coli
Lactococcin-A immunity protein Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 11.18 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3M7 (Residues: 1-98; Coverage: 100%)
Gene name: lciA
Sequence domains: Enterocin A Immunity
Bacteriocin lactococcin-A Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 5.78 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A312 (Residues: 22-75; Coverage: 72%)
Gene name: lcnA
Sequence domains: Lactococcin-like family

Ligands and Environments

1 bound ligand:
Ligand MAN 3 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.98Å
Relevant EMDB volumes: EMD-34726
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacteriocin diversity, function, discovery and application as antimicrobials.
Sugrue et al. (2024)

3 mentions without citation

Structural Basis of the Mechanisms of Action and Immunity of Lactococcin A, a Class IId Bacteriocin.
Li et al. (2023)
2 more