› 8hms

X-ray diffraction
2.1Å resolution

Crystal Structure of PKM2 mutant C474S

Released:
DOI: 10.2210/pdb8hms/pdb
PDB entry 8hms coloured by chain, front view.
PDB entry 8hms coloured by chain, side view.
PDB entry 8hms coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Upadhyay S, Kumar A, Patel AK

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 60.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand FBP 3 x FBP
Ligand OXL 4 x OXL
Ligand EDO 7 x EDO
Ligand MG 5 x MG
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE PX-BL21
Spacegroup: P212121
Unit cell:
a: 98.733Å b: 134.584Å c: 148.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.238
Expression system: Escherichia coli

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