› 8hrh

X-ray diffraction
2.07Å resolution

SN-131/1B2 anti-MUC1 antibody with a glycopeptide

Released:
DOI: 10.2210/pdb8hrh/pdb
PDB entry 8hrh coloured by chain, front view.
PDB entry 8hrh coloured by chain, side view.
PDB entry 8hrh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural and molecular insight into antibody recognition of dynamic neoepitopes in membrane tethered MUC1 of pancreatic cancer cells and secreted exosomes.
Wakui H, Yokoi Y, Horidome C, Ose T, Yao M, Tanaka Y, Hinou H, Nishimura SI
OpenAccess logo RSC Chem Biol 4 564-572 (2023)
PMID: 37547453

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227479 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy chain of SN-131/1B2 antibody Fab Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 51.47 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Light chain of SN-131/1B2 antibody Fab Chain: B
Molecule details ›
Chain: B
Length: 238 amino acids
Theoretical weight: 26.66 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL, SIA
Carbohydrate polymer
10 bound ligands:
Ligand N7P 1 x N7P
Ligand ASP 1 x ASP
Ligand THR 1 x THR
Ligand ARG 1 x ARG
Ligand ALA 1 x ALA
Ligand TRS 1 x TRS
Ligand PEG 7 x PEG
Ligand GOL 2 x GOL
Ligand PRO 1 x PRO
Ligand LPD 1 x LPD
1 modified residue:
Ligand MLY 20 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P6522
Unit cell:
a: 84.59Å b: 84.59Å c: 271.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.226
Expression system: Mus musculus

Quick links

Citations

1 mention without citation

Structural and molecular insight into antibody recognition of dynamic neoepitopes in membrane tethered MUC1 of pancreatic cancer cells and secreted exosomes.
Wakui et al. (2023)