› 8iax

X-ray diffraction
1.8Å resolution

Crystal structure of Streptococcus pneumoniae pyruvate kinase in complex with phosphoenolpyruvate and fructose 1,6-bisphosphate

Released:
DOI: 10.2210/pdb8iax/pdb
PDB entry 8iax coloured by chain, front view.
PDB entry 8iax coloured by chain, side view.
PDB entry 8iax coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
Functional and structural characterization of Streptococcus pneumoniae pyruvate kinase involved in fosfomycin resistance.
Taguchi A, Nakashima R, Nishino K
OpenAccess logo J Biol Chem 104892 (2023)
PMID: 37286036

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 521 amino acids
Theoretical weight: 56.99 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DQ84 (Residues: 1-501; Coverage: 100%)
Gene names: pykF, spr0797
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand FBP 8 x FBP
Ligand PEP 8 x PEP
Ligand MG 8 x MG
Ligand K 8 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 221.061Å b: 86.644Å c: 258.148Å
α: 90° β: 100.86° γ: 90°
R-values:
R R work R free
0.184 0.183 0.221
Expression system: Escherichia coli

Quick links

Citations

1 review citation

What is allosteric regulation? Exploring the exceptions that prove the rule!
McCullagh et al. (2024)

1 mention without citation

Functional and structural characterization of Streptococcus pneumoniae pyruvate kinase involved in fosfomycin resistance.
Taguchi et al. (2023)